Download Advanced Photon Counting: Applications, Methods, by Peter Kapusta, Michael Wahl, Rainer Erdmann PDF

By Peter Kapusta, Michael Wahl, Rainer Erdmann

This quantity makes a speciality of Time-Correlated unmarried Photon Counting (TCSPC), a robust device permitting luminescence lifetime measurements to be made with excessive temporal solution, even on unmarried molecules. Combining spectrum and lifelong presents a “fingerprint” for opting for such molecules within the presence of a heritage. Used including confocal detection, this allows single-molecule spectroscopy and microscopy as well as ensemble measurements, starting up an incredible diversity of scorching lifestyles technology purposes corresponding to fluorescence lifetime imaging (FLIM) and size of Förster Resonant strength move (FRET) for the research of protein folding and interplay. numerous technology-related chapters current either the fundamentals and present cutting-edge, specifically of TCSPC electronics, photon detectors and lasers. the rest chapters conceal a large diversity of functions and methodologies for experiments and knowledge research, together with the lifestyles sciences, illness facilities in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new techniques bobbing up from the mix of vintage TCSPC and fluorescence lifetime with tools in accordance with depth fluctuation signify a very specified highlight.

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6 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 32 32 32 32 33 33 34 34 35 35 37 38 38 40 40 41 1 General Introduction Detectors are core components in every setup based on photon counting. For the spectral range between approx.

3. Afterpulsing – afterpulsing refers to additional, artificial output signals that are not related to a photon detection event but correlated to a previous detection event. In time-resolved measurements, afterpulses are visible as additional signal peaks at a defined temporal spacing to the main signal. Afterpulses are Single-Photon Counting Detectors for the Visible Range Between 300 and 1,000 nm 25 generated “inside” the detector and cannot be avoided or removed. Afterpulsing is usually expressed as a percentage that expresses the probability that one detected photon creates an afterpulsing event.

5 Geometrical Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Hybrid PMTs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 General Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Detection Efficiency . . . . . . .

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