By F. Palmieri, G. Genchi, V. Zara, C. Indiveri, F. Bisaccia (auth.), Prof. Dr. Angelo Azzi, Dr. Katarzyna A. Nałęz, Dr. Maciej J. Nałęcz, Dr. Lech Wojtczak (eds.)
In order to supply a practical employer of many of the biochemical procedures, the intracellular atmosphere is subdivided into booths shaped by means of organelles. Mitochondria serve to revive energy-rich compounds, whereas power is especially fed on within the cytosolic house. Molecules eager about those metabolic cycles need to pass the mitochondrial membrane through really good providers embedded within the internal mitochondrial membrane. All correct structural and sensible points of the Anion vendors of Mitochondrial Membranes in addition to isolation methods, reconstitution experiments and biogenesis of the vendors, kinetics of delivery in addition to the metabolic legislation mechanisms are handled during this volume.
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Extra info for Anion Carriers of Mitochondrial Membranes
The celite eluates, containing still a mixture of different proteins (Fig. 2), were processed further by chromatography on organo-mercurial agarose (Affigel 501). This last step was carried out in a batch-procedure for the appropriate incubation time as described in Material and Methods. 5-32 kDa was not bound to the resin as shown in Fig. 2. The same result was obtained starting either from heart or liver mitochondria. , 1988. All other proteins were bound by Affigel 501 and could be eluted with 200 mM 2-mercaptoethanol (not shown).
For the exchange experiments media used for reconstitution were supplemented with 50 mM substrate. 5-2 h at 25°C and the process was stopped by removing Amberlite and the external medium by passing through Dowex-CI columns equilibrated with 170 mM sucrose. 2 for exchange experiments and to 8 for uptake measurements. 2 mM outside) followed the procedure described by Bisaccia et aL (1985). Measurements of exchange activity of the pyruvate carrier were performed at 15°C as described by Na,r~cz et aL (1986).
Nature 183:1657-1659 Riccio P, Aquila H, Klingenberg M (1975a) Solubilization of the carboxyatractylatebinding protein from mitochondna. FEllS Lett. 56: 129-132. Riccio P, Aquila H, Klingenberg M (1975b) Purification of the carboxyatractylatebinding protein from mitochondria. ' purification by hydroxyapatite chromato~raphy in Triton X-toO, polypeptide pattern and respiratory chain characteristics. Blochim Biophys Acta 459:250-262 Riccio P (1983) Adsorption chromatography of proteins in nonionic detergents.